FM Minimal medium w/o Cysteine and w/o Methionine Size:1000g aminoglycosides bind to the 30S

aminoglycosides bind to the 30S and to some extent to the 50 S -subunits of the bacterial ribosome

Soluble in water

6 (20 mN) Tryptone 20 Yeast extract 5 NaCl 0

Chemical formula C4H9NO3 Solubility (20° C) 90 g/l Mol

FM Minimal medium w/o Cysteine and w/o Methionine Size:1000g aminoglycosides bind to the 30SThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to